In vivo shuffling via heteroduplex amplicons

Bluescreen transilluminator photo showing
different shades of green.

In a previous post I discuss the heteroduplicity of epPCR plasmids. Namely often colonies in a library will have bases that have two equal variants. The most likely cause being that the amplicons do not anneal perfect and that the transformed plasmids are actually heteroduplexes. These can either divide before being corrected or are corrected by mismatch repair.
I did a sneaky experiment to see if this could be used to make protocol to shuffle mutations between variants and got a positive result, but possibly not as effective as hoped.

Fluorescent fats

Desaturated fats generally have a methylene-interrupted configuration, where a double bond is separated by two single bonds. Conjugated fatty acids (aka. polyene fatty acids) are fluorescent when in a lipid bilayer (Sklar et al., 1977), but few examples are know.

One example is parinaric acid (octadecatetraenoic acid; pictured) from Impatiens balsamina (also found in the unrelated plant Parinarium laurinum; this is not a former name for Impatiens balsamina) with four double bonds (ex. 320 nm → em. 420 nm) and with a characterised conjugase (Cahoon et al., 1999). Other conjugases have been studied (Rawat et al., 2012) and other conjugated fatty acids are known, including one with 5 double bonds (bosseopentaenoic (eicosapentaenoic) acid).
A single enzyme that makes a fluorescent reportable signal is rather appealing for synthetic biology. 
The wavelength is rather limiting compared to aromatic compounds obviously. Namely conjugated butadiene, hexatriene, octatetraene, decapentaene  and dodecahexaene absorb at 217 nm, 252 nm, 304 nm, 324 and 340 nm respectively. The fact that two or three conjugated ene bonds would not suffice as a tool raises the question of whether a conjugase could be evolved to make even more conjugated systems, such one acting on cervonic acid (22:6(n-3)), which would make a fluorophore of seven conjugated ene bonds. As a fluorescent signal is easily selectable by FACS, it would be definitely doable (if the UV lasers were available). The only problem is that E. coli lacks variety when it comes to membrane facts as is mainly composed of palmitic (hexadecanoic) acid, palmitoleic (cis-9-hexadecenoic) acid and cis-vaccenic (cis-11-octadecenoic) acid (Mansilla et al., 2004), therefore, the desaturation machiner to make the precursors would be needed. Nevertheless, it is rather interesting and I would love to see more colourful E. coli...

EDIT. Isoprenoid and is condensed derivatives (geranyl-PP, farnesyl-PP, geranylgeranyl-PP etc.) already have a methylene-interrupted configuration and geranylgeranyl is condensed into phytoene which is desaturated by phytoene dehydrogenase to lycopene.

Archaea use diphytanylglyceryl phosphoglycerol is their cell membranes: phytanol is reduced geranylgeranol. So their reduce it as opposed to desaturating it, if they did you'd 5-7 conjugated ene bonds, which would absorb in the yellow region.