I recently was asked a straightforward task: get the energy scores of a given protein by itself, conjugated with fluorescein maleimide and its ring opened form. Despite that, I failed miserably because of implicit waters playing odd tricks.
Given that it is of no real use, I am happy to share so to give a tutorial of how to do that in Rosetta (ligand docking), which I found taxing the first time.
A sticky mess of disconnected ideas, analyses and just plain silly posts about computational biochemistry, synthetic biology and microbiology.
Monday, 19 March 2018
Sunday, 18 March 2018
GFP is technically a suicide enzyme
GFP has an oxygen dependent maturation step to form its cofactor. Therefore, it could be farcically considered a suicide enzyme...
It performs a reaction: it converts oxygen to peroxide —doing a single turnover.
Subscribe to:
Posts (Atom)