The distribution of mutations in an error prone library has been modelled in Sun (1995) based on the underlying principle of a PCR reaction. In the paper it is found that the distribution becomes more and more similar to a regular Possion and the two are virtually the same after 10 cycles. However, given that one most likely does not know what one's PCR efficiency is, using this formula may be dangerous. Whereas in reality getting a better estimate of the mean number of mutations may be more helpful.
A segfault and NaN driven series of disconnected ideas, analyses and just plain silly posts about computational biochemistry, synthetic biology and microbiology.
Saturday 25 August 2018
Sunday 12 August 2018
Protein dropcaps
To make a webpage stylish does not mean that it cannot be biochemically themed. In fact, to can have both thanks to protein dropcaps! using the protein alphabet made by Prof. Mark Howarth in Nature Structural and Molecular Biology in May 2015(available Here) in combination with a wee JS script I wrote you can be a stylish biochemist. The script along with documentation is available in my GitHub page: https://github.com/matteoferla/protein_alphabet_dropcaps.
Saturday 4 August 2018
A fully suicidal enzyme follows a second order equation
That a fully suicidal enzyme follows a second order equation is a rather intuitive statement, but it is nice to have formal proof, which is as follows.
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