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Monday, 19 March 2018

The curious case of fluorescein and implicit waters

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I recently was asked a straightforward task: get the energy scores of a given protein by itself, conjugated with fluorescein maleimide and i...
Sunday, 18 March 2018

GFP is technically a suicide enzyme

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GFP has an oxygen dependent maturation step to form its cofactor. Therefore, it could be farcically considered a suicide enzyme... It perfor...
Saturday, 6 January 2018

Skewed mutational biases: English scrabble with Klingon tiles

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Skewed mutational biases: English scrabble with Klingon tiles In a game of Scrabble one draws seven tiles from a bag and the best result...
2 comments:
Thursday, 4 January 2018

Pi projects: a new year's resolutions

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2018 is here. I have papers that I want to or have to write and endless tasks I want to complete, but life is not all about career-driven se...
Monday, 16 October 2017

Hacking PDBs for fusion protein and missing loops

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This is a How-to guide to make structures of fusion protein —the shoddy way. For the proper way see the how to do it in Rosetta or Pyroset...
1 comment:
Friday, 18 August 2017

Rosetta easteregg

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The guide to use Rosetta may be a bit, ehm, flaky, but I think it is made up for by this rather amusing comment header I found in one of its...
Wednesday, 5 July 2017

In vivo shuffling via heteroduplex amplicons

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Bluescreen transilluminator photo showing different shades of green. In a previous post I discuss the heteroduplicity of epPCR plasmids...