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Thursday, 25 February 2016

Medical diagnosis with Mutazyme

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A cornerstone enzyme for error-prone PCR is Mutazyme, an enzyme with an increase error rate, but less biased than manganese mutagenesis. The...
Monday, 15 February 2016

Biochemical reaction yield and enzyme promiscuity

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Reaction yield,  i.e.  the molar percentage of product over substrate, is often mentioned by chemists, but never by biochemists. My guess is...
Saturday, 6 February 2016

Promiscuously hitchhiking on a pathway

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In the seminal 1976 paper on enzyme evolution Roy Jensen first pointed out that the TCA cycle, the ketoadipate route to lysine, pantothenate...
Sunday, 17 January 2016

Uncultured bacterial majority? Digitally unannotated majority of the minority is worse

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Aquifex , an exiting bacterium, which unexciting BioSample data. It has been remarked that the majority of bacterial diversity remains u...
Saturday, 16 January 2016

The contagious ORF annotation error of 16S rRNA

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Some time back in many genomes there were a few copies of a small hypothetical open reading frame, sometimes annotated as a quinone oxidase....
Saturday, 5 December 2015

The future of enzymology?

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EDIT: I called it! Turns out this was much closer to reality than I thought and a paper came out doing exactly this. Assaying enzymes is ...
Saturday, 28 November 2015

ABS biosynthesis

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Lego, rumour has it, wants to biosynthesise acrylonitrile, butadiene styrene (ABS), the resin that gives their blocks their firm hold and t...